The Human AGRN (Agrin) CLIA Kit is a highly sensitive and specific assay designed for the precise measurement of agrin levels in human samples, including serum, plasma, and cell culture supernatants. This kit provides accurate and reliable results, making it suitable for various research applications.Agrin is a critical protein that plays a vital role in neuromuscular junction formation and function, as well as in the regulation of synaptic plasticity.
Dysregulation of agrin levels has been linked to various neurological disorders, making it a valuable biomarker for studying these conditions and developing potential therapies.Overall, the Human AGRN (Agrin) CLIA Kit is an essential tool for researchers looking to investigate the role of agrin in neurological disorders and explore its potential as a therapeutic target.
Product Name:
Human AGRN (Agrin) CLIA Kit
SKU:
HUES00219
Target:
Human AGRN (Agrin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human AGRN. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AGRN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AGRN, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human AGRN. You can calculate the concentration of Human AGRN in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-96
91-107
92-106
Average (%)
91
97
98
1:4
Range (%)
96-112
93-106
92-102
Average (%)
102
99
97
1:8
Range (%)
98-114
99-116
96-112
Average (%)
105
107
104
1:16
Range (%)
89-104
90-102
94-110
Average (%)
96
96
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-110
100
EDTA plasma (n=5)
88-104
96
Cell culture media (n=5)
92-108
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
107.02
228.85
745.59
102.8
226.55
806.41
Standard deviation
13.58
22.13
72.62
11.3
23.56
52.09
C V (%)
12.69
9.67
9.74
10.99
10.4
6.46
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human AGRN concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human AGRN in samples. No significant cross-reactivity or interference between Human AGRN and analogues was observed.
