Description
Human AgRP (Agouti Related Protein) ELISA Kit
The AgRP ELISA Kit is uniquely crafted for the precise quantitative detection of Agouti-Related Protein (AgRP) levels in various human biological samples. AgRP, a crucial regulator of feeding behavior and energy homeostasis, plays a significant role in appetite modulation and energy regulation. Quantifying AgRP accurately is essential for understanding its role in metabolic processes, obesity, and related disorders, offering insights into potential therapeutic approaches and disease mechanisms.
Our AgRP ELISA Kit boasts exceptional sensitivity and specificity, ensuring accurate and reproducible results. Manufactured under rigorous quality control measures, this kit guarantees reliable performance and user-friendly protocols suitable for both research and clinical applications. Rely on our AgRP ELISA Kit for dependable and precise quantification of this vital biomarker in your studies and research endeavors.
| Product Name: | Human AgRP (Agouti Related Protein) ELISA Kit |
| Product Code: | AEES00043 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Human |
| Detection Range: | 7.81-500 pg/mL |
| Sensitivity: | 4.69 pg/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 100?L |
| Specificity: | This kit recognizes Human AgRP in samples. No significant cross-reactivity or interference between Human AgRP and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Human AgRP concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human AgRP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AgRP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AgRP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human AgRP. You can calculate the concentration of Human AgRP in the samples by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
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| 1. | Add 100?L standard or sample to the wells. Incubate for 90 min at 37°C |
| 2. | Discard the liquid, immediately add 100?L Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
| 3. | Aspirate and wash the plate for 3 times |
| 4. | Add 100?L HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 5. | Add 90?L Substrate Reagent. Incubate for 15 min at 37°C |
| 6. | Add 50?L Stop Solution |
| 7. | Read the plate at 450nm immediately. Calculation of the results |
