The Human AHR (Aryl Hydrocarbon Receptor) CLIA Kit is a highly reliable and sensitive assay designed for the accurate detection of aryl hydrocarbon receptor levels in human biological samples. This kit is ideal for researchers studying the role of AHR in various health conditions, including cancer, metabolic disorders, and environmental toxicities.The aryl hydrocarbon receptor is a key regulator of gene expression in response to environmental toxins and plays a crucial role in the detoxification of harmful compounds. By measuring AHR levels in serum, plasma, or cell culture supernatants, researchers can gain valuable insights into the mechanisms underlying these conditions and potentially identify new therapeutic targets.
With its superior sensitivity and specificity, the Human AHR CLIA Kit offers reliable and reproducible results, making it a valuable tool for biomedical research. Whether investigating the impact of pollutants on human health or studying the molecular pathways of disease, this kit provides precise measurements to advance scientific understanding in the field.
Product Name:
Human AhR (Aryl Hydrocarbon Receptor) CLIA Kit
SKU:
HUES00360
Target:
Human AhR (Aryl Hydrocarbon Receptor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human AhR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AhR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AhR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human AhR. You can calculate the concentration of Human AhR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-115
85-98
86-99
Average (%)
106
91
91
1:4
Range (%)
100-117
95-105
95-111
Average (%)
107
100
103
1:8
Range (%)
98-115
103-120
98-113
Average (%)
106
110
106
1:16
Range (%)
91-105
94-108
84-98
Average (%)
98
101
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-111
104
EDTA plasma (n=5)
100-116
107
Cell culture media (n=5)
98-112
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
260.65
737.59
2444.15
256.59
763.1
2647.18
Standard deviation
33.62
82.54
187.22
26.76
66.16
294.9
C V (%)
12.9
11.19
7.66
10.43
8.67
11.14
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human AhR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human AhR in samples. No significant cross-reactivity or interference between Human AhR and analogues was observed.
