Human AIF1 (Allograft Inflammatory Factor 1) CLIA Kit
The Human Allograft Inflammatory Factor 1 (AIF1) CLIA Kit is specifically designed for the precise and accurate detection of AIF1 levels in human samples including serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, ensuring reliable and reproducible results that are essential for a variety of research applications.AIF1, also known as allograft inflammatory factor 1, is a critical protein involved in immune response and inflammation. It plays a key role in regulating inflammatory processes and immune cell activation, making it a valuable biomarker for studying autoimmune disorders, inflammatory diseases, and other related conditions.
Understanding AIF1 levels can provide insight into disease progression and potential therapeutic targets.With the Human AIF1 CLIA Kit, researchers can accurately measure AIF1 levels in human samples, advancing our understanding of immune response and inflammation-related diseases. Whether studying autoimmune disorders, inflammatory diseases, or other related conditions, this kit offers a reliable and effective solution for investigating the role of AIF1 in various biological processes.
Product Name:
Human AIF1 (Allograft Inflammatory Factor 1) CLIA Kit
SKU:
HUES00221
Target:
Human AIF1 (Allograft Inflammatory Factor 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human AIF1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AIF1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AIF1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human AIF1. You can calculate the concentration of Human AIF1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-111
86-99
84-96
Average (%)
105
91
91
1:4
Range (%)
88-101
100-114
90-101
Average (%)
94
107
96
1:8
Range (%)
94-108
85-97
93-105
Average (%)
100
90
100
1:16
Range (%)
95-108
96-113
88-101
Average (%)
101
103
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
95
EDTA plasma (n=5)
85-100
92
Cell culture media (n=5)
101-115
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
37.45
82.23
389.36
40.07
85.45
384.98
Standard deviation
4.36
6.46
40.42
3.94
7.58
38.84
C V (%)
11.64
7.86
10.38
9.83
8.87
10.09
Sample type &Sample volume:
Tissue homogenates,cell lysates and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human AIF1 concentrations in Tissue homogenates,cell lysates and other biological fluids.
Specificity:
This kit recognizes Human AIF1 in samples. No significant cross-reactivity or interference between Human AIF1 and analogues was observed.
