The Human AIRE (Autoimmune Regulator) CLIA Kit is a highly sensitive and specific assay designed for the precise measurement of AIRE levels in human samples such as serum and plasma. This innovative kit offers reliable and reproducible results, making it an indispensable tool for researchers studying autoimmune disorders and immuno-oncology. The Autoimmune Regulator (AIRE) protein plays a critical role in maintaining immune tolerance by regulating the expression of self-antigens in the thymus. Dysfunction of AIRE has been linked to various autoimmune diseases, including autoimmune polyendocrine syndrome type 1 (APS-1).
By accurately measuring AIRE levels, researchers can gain valuable insights into autoimmune mechanisms and potentially develop targeted therapies for these debilitating conditions. With its high performance and accuracy, the Human AIRE CLIA Kit is a valuable asset for immunologists, oncologists, and researchers exploring the intricate mechanisms of autoimmunity. Order yours today from Assaygenie and advance your research with confidence.
Product Name:
Human AIRE (Autoimmune Regulator) CLIA Kit
SKU:
HUES00374
Target:
Human AIRE (Autoimmune Regulator)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human AIRE. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AIRE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AIRE, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human AIRE. You can calculate the concentration of Human AIRE in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-102
100-115
101-117
Average (%)
93
106
109
1:4
Range (%)
100-119
84-97
102-120
Average (%)
109
91
110
1:8
Range (%)
91-104
98-111
85-97
Average (%)
98
106
90
1:16
Range (%)
99-115
85-99
84-99
Average (%)
105
91
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-106
100
EDTA plasma (n=5)
92-103
97
Cell culture media (n=5)
91-104
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
100.52
262.77
966.93
100.87
281.12
972.0
Standard deviation
9.2
24.49
101.62
8.43
27.83
107.31
C V (%)
9.15
9.32
10.51
8.36
9.9
11.04
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human AIRE concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human AIRE in samples. No significant cross-reactivity or interference between Human AIRE and analogues was observed.
