Human Aldolase A, Fructose Bisphosphate (ALDOA) ELISA Kit
The Human Aldolase A (Fructose-bisphosphate aldolase A) ELISA Kit offered by AssayGenie is specifically designed for the accurate detection of aldolase A levels in human serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit ensures reliable and reproducible results, making it an ideal tool for a wide range of research applications.Aldolase A is a key enzyme involved in glycolysis, playing a crucial role in energy production and metabolism. Dysregulation of aldolase A has been implicated in various diseases, including cancer, metabolic disorders, and neurodegenerative conditions.
Therefore, monitoring aldolase A levels can provide valuable insights into disease progression and potential therapeutic interventions.By using the Human Aldolase A ELISA Kit from AssayGenie, researchers can accurately quantify aldolase A levels in biological samples, allowing for in-depth investigations into its role in health and disease. With its user-friendly protocol and reliable performance, this kit is an essential tool for advancing research in the field of biochemistry and molecular biology.
Product Name:
Human Aldolase A, Fructose Bisphosphate (ALDOA) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
