Human Alpha-2-Heremans Schmid Glycoprotein (aHSG) ELISA Kit
The Human Alpha-2-Heremans-Schmid Glycoprotein (AHSG) ELISA Kit is a powerful tool for the accurate quantification of AHSG levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, guaranteeing precise and dependable results for a variety of research applications.AHSG, also known as fetuin-A, is a multifunctional glycoprotein that plays a crucial role in regulating various physiological processes, including inflammation, insulin resistance, and calcium homeostasis. Dysregulation of AHSG has been implicated in several diseases such as diabetes, cardiovascular disorders, and liver diseases, highlighting its potential as a valuable biomarker for disease diagnosis and monitoring.
With its user-friendly protocol and reliable performance, the Human AHSG ELISA Kit is an essential tool for researchers and clinicians working in the fields of biomarker discovery, disease diagnosis, and drug development. Trust in this kit to provide accurate and reproducible results, advancing your research efforts and paving the way for improved healthcare outcomes.
Product Name:
Human Alpha-2-Heremans Schmid Glycoprotein (aHSG) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
