The Human Alpha-1B Glycoprotein (A1BG) CLIA Kit is specifically designed for the accurate measurement of alpha-1B glycoprotein levels in human samples such as serum and plasma. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.Alpha-1B glycoprotein is a key protein involved in various biological processes, including immune response regulation and cell adhesion.
Abnormal levels of A1BG have been associated with certain diseases and conditions, making it an important biomarker for research and potential diagnostic purposes.With easy-to-follow protocols and quick assay turnaround times, the Human A1BG CLIA Kit is a valuable tool for researchers studying the role of alpha-1B glycoprotein in health and disease. Get accurate and consistent results with this high-quality assay kit from Assay Genie.
Product Name:
Human alpha1BG (Alpha-1-B-Glycoprotein) CLIA Kit
SKU:
HUES00571
Target:
Human alpha1BG (Alpha-1-B-Glycoprotein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human α1BG. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human α1BG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human α1BG, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human α1BG. You can calculate the concentration of Human α1BG in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-109
88-101
104-120
Average (%)
103
93
110
1:4
Range (%)
91-104
89-103
96-114
Average (%)
98
96
104
1:8
Range (%)
96-111
104-121
88-98
Average (%)
104
110
93
1:16
Range (%)
96-109
83-98
89-103
Average (%)
102
90
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-109
103
EDTA plasma (n=5)
100-116
106
Cell culture media (n=5)
86-100
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
415.6
707.76
3397.04
400.83
659.24
3325.46
Standard deviation
41.06
72.62
347.18
37.72
72.71
215.16
C V (%)
9.88
10.26
10.22
9.41
11.03
6.47
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human α1BG concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human α1BG in samples. No significant cross-reactivity or interference between Human α1BG and analogues was observed.
