The Human Alpha-2-Macroglobulin (alpha2-M) CLIA Kit is a powerful tool for the precise quantification of alpha2-M levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, delivering accurate and consistent results that are perfect for a variety of research applications.Alpha2-Macroglobulin is a key protein involved in numerous physiological processes, including the regulation of inflammation, immune response, and wound healing.
With its crucial role in conditions like liver disease, arthritis, and Alzheimer's disease, alpha2-M serves as a valuable biomarker for understanding disease mechanisms and investigating potential treatment targets.Overall, the Human Alpha-2-Macroglobulin CLIA Kit is an essential tool for researchers seeking to explore the intricate functions of alpha2-M and its involvement in various diseases, paving the way for innovative advancements in diagnostics and therapeutics.
Product Name:
Human alpha2-M (Alpha-2 Macroglobulin) CLIA Kit
SKU:
HUES00574
Target:
Human alpha2-M (Alpha-2 Macroglobulin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human α2-M. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human α2-M and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human α2-M, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human α2-M. You can calculate the concentration of Human α2-M in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-109
101-117
95-107
Average (%)
99
109
102
1:4
Range (%)
94-109
103-119
101-113
Average (%)
102
109
107
1:8
Range (%)
86-101
95-110
93-104
Average (%)
93
102
98
1:16
Range (%)
87-98
86-100
102-115
Average (%)
92
92
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-115
107
EDTA plasma (n=5)
89-102
95
Cell culture media (n=5)
99-113
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.49
1.23
4.49
0.51
1.25
4.38
Standard deviation
0.04
0.14
0.4
0.06
0.13
0.46
C V (%)
8.16
11.38
8.91
11.76
10.4
10.5
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human α2-M concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human α2-M in samples. No significant cross-reactivity or interference between Human α2-M and analogues was observed.
