Human AMPK (Phosphorylated Adenosine Monophosphate Activated Protein Kinase) CLIA Kit
The Human AMPK (Phosphorylated Adenosine Monophosphate-Activated Protein Kinase) CLIA Kit is specifically designed for the quantitative detection of phosphorylated adenosine monophosphate-activated protein kinase levels in human serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, providing accurate and reproducible results for a variety of research applications.AMPK is a critical enzyme that plays a central role in regulating cellular energy homeostasis. It functions as a metabolic master switch, regulating various cellular processes such as glucose uptake, fatty acid oxidation, and protein synthesis.
Dysregulation of AMPK has been implicated in various metabolic disorders, including obesity, type 2 diabetes, and cardiovascular disease.By accurately measuring phosphorylated AMPK levels, researchers can gain valuable insights into the metabolic status of cells and tissues, as well as potential therapeutic targets for metabolic diseases. Order the Human AMPK CLIA Kit today to advance your research in the fields of metabolism, energy regulation, and metabolic diseases.
Product Name:
Human AMPK (Phosphorylated Adenosine Monophosphate Activated Protein Kinase) CLIA Kit
SKU:
HUES00231
Target:
Human AMPK (Phosphorylated Adenosine Monophosphate Activated Protein Kinase)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human AMPK. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AMPK and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AMPK, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human AMPK. You can calculate the concentration of Human AMPK in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-101
85-100
89-106
Average (%)
93
92
97
1:4
Range (%)
87-102
94-111
87-98
Average (%)
93
101
93
1:8
Range (%)
90-101
90-103
98-112
Average (%)
95
97
106
1:16
Range (%)
90-105
97-111
100-116
Average (%)
97
102
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-113
103
EDTA plasma (n=5)
90-104
98
Cell culture media (n=5)
89-99
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
222.17
342.01
1651.96
238.78
369.06
1714.28
Standard deviation
18.88
27.09
179.07
21.37
42.04
196.8
C V (%)
8.5
7.92
10.84
8.95
11.39
11.48
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human AMPK concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human AMPK in samples. No significant cross-reactivity or interference between Human AMPK and analogues was observed.
