Human AMY2 (Amylase Alpha 2, Pancreatic) ELISA Kit
The Human Alpha-2 Amylase ELISA Kit is a reliable tool for the precise measurement of alpha-2 amylase levels in human samples such as serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.Alpha-2 amylase is an enzyme primarily produced in the pancreas and salivary glands, playing a key role in the digestion of carbohydrates. Abnormal levels of alpha-2 amylase have been associated with various health conditions, including pancreatic disorders and diabetes, making it a valuable biomarker for studying these diseases and exploring potential treatment options.
With its easy-to-use format and proven performance, the Human Alpha-2 Amylase ELISA Kit is an essential tool for researchers and clinicians seeking to investigate the role of alpha-2 amylase in health and disease.
Product Name:
Human AMY2 (Amylase Alpha 2, Pancreatic) ELISA Kit
SKU:
HUES03121
Target:
Human AMY2 (Amylase Alpha 2, Pancreatic)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human AMY2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AMY2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AMY2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human AMY2. You can calculate the concentration of Human AMY2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-111
94-110
90-102
Average (%)
101
102
96
1:4
Range (%)
88-100
83-95
84-96
Average (%)
94
88
89
1:8
Range (%)
93-107
86-98
89-102
Average (%)
100
91
94
1:16
Range (%)
92-104
81-92
87-101
Average (%)
97
87
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-98
90
EDTA plasma (n=5)
96-110
102
Cell culture media (n=5)
90-104
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.26
24.47
72.49
9.2
23.43
70.2
Standard deviation
0.65
1.03
2.72
0.64
1.16
2.54
C V (%)
7.02
4.21
3.75
6.96
4.95
3.62
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human AMY2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human AMY2 in samples. No significant cross-reactivity or interference between Human AMY2 and analogues was observed.
