The Human ANG (Angiostatin) CLIA Kit is a highly reliable and efficient tool for measuring levels of angiostatin in human samples such as serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit ensures accurate and reproducible results, making it an invaluable asset for a variety of research applications.Angiostatin is a key regulator of angiogenesis, the process of blood vessel formation, and is involved in influencing cell proliferation.
Its role in diseases such as cancer, cardiovascular conditions, and neurodegenerative disorders underscores its importance as a biomarker for studying these illnesses and developing potential therapies. Trust the Human ANG (Angiostatin) CLIA Kit to provide precise and insightful data for your research needs.
Product Name:
Human ANG (Angiostatin) CLIA Kit
SKU:
HUES00234
Target:
Human ANG (Angiostatin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
3.75 ng/mL
Detection range:
6.25-400 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ANG, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ANG. You can calculate the concentration of Human ANG in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-99
84-99
96-111
Average (%)
94
90
103
1:4
Range (%)
86-102
103-121
94-106
Average (%)
93
110
99
1:8
Range (%)
97-113
97-110
88-101
Average (%)
103
104
93
1:16
Range (%)
97-110
103-118
96-110
Average (%)
102
110
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-106
100
EDTA plasma (n=5)
88-98
93
Cell culture media (n=5)
94-108
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
21.3
35.31
176.34
19.24
36.11
185.79
Standard deviation
2.44
3.98
11.37
1.95
2.59
21.29
C V (%)
11.46
11.27
6.45
10.14
7.17
11.46
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ANG concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ANG in samples. No significant cross-reactivity or interference between Human ANG and analogues was observed.
