The Human ANG (Angiostatin) ELISA Kit is meticulously crafted for the precise measurement of Angiostatin (ANG) levels in diverse biological samples. ANG, a potent endogenous angiogenesis inhibitor cleaved from plasminogen, exhibits crucial regulatory functions in the control of blood vessel formation and tumor growth. Its multifaceted roles in inhibiting the proliferation of endothelial cells and modulating angiogenesis processes make it a pivotal player in vascular biology, cancer research, and therapeutic interventions.
Our ANG ELISA Kit delivers outstanding sensitivity and specificity, ensuring the generation of accurate and reproducible data. Engineered with excellence and adherence to stringent quality standards, this kit is designed to provide robust performance, making it the optimal choice for cutting-edge research applications. Rely on Assay Genie's ANG ELISA Kit for the reliable and precise quantification of this essential protein in your scientific explorations.
Product Name:
Human ANG (Angiostatin) ELISA Kit
SKU:
AEES00168
Target:
Human ANG (Angiostatin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ANG. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ANG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ANG. You can calculate the concentration of Human ANG in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-107
92-106
92-105
Average (%)
99
98
100
1:4
Range (%)
90-101
92-106
96-110
Average (%)
95
97
101
1:8
Range (%)
89-104
93-106
92-102
Average (%)
95
99
97
1:16
Range (%)
90-107
88-100
89-101
Average (%)
97
95
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-107
97
EDTA plasma (n=5)
94-105
100
Cell culture media (n=5)
94-110
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
97.29
304.43
989.12
101.99
314.65
983.65
Standard deviation
6.22
15.13
33.33
6.21
13.37
34.33
C V (%)
6.39
4.97
3.37
6.09
4.25
3.49
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ANG concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ANG in samples. No significant cross-reactivity or interference between Human ANG and analogues was observed.
