Human ANG II R1 (Angiotensin II Receptor 1) ELISA Kit
The Human Angiotensin II Receptor 1 (ANG II R1) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of ANG II R1 levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it suitable for a variety of research applications.The ANG II R1 is a key receptor involved in the renin-angiotensin system, which plays a crucial role in regulating blood pressure and fluid balance.
Dysregulation of the system has been implicated in various cardiovascular and renal diseases. By measuring ANG II R1 levels, researchers can gain valuable insights into these conditions and potential therapeutic interventions.Overall, the Human ANG II R1 ELISA Kit offers researchers a powerful tool for studying the renin-angiotensin system and its role in health and disease. Get accurate and reliable results with this advanced ELISA kit from Assay Genie.
Product Name:
Human ANG II R1 (Angiotensin II Receptor 1) ELISA Kit
SKU:
HUES01549
Target:
Human ANG II R1 (Angiotensin II Receptor 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ANGⅡR1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANGⅡR1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ANGⅡR1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ANGⅡR1. You can calculate the concentration of Human ANGⅡR1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-98
88-102
87-99
Average (%)
92
94
93
1:4
Range (%)
88-99
86-98
84-98
Average (%)
94
93
90
1:8
Range (%)
94-106
85-98
86-97
Average (%)
99
91
92
1:16
Range (%)
91-103
83-99
88-102
Average (%)
96
90
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-107
98
EDTA plasma (n=5)
95-106
100
Cell culture media (n=5)
96-111
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.52
1.03
4.33
0.53
1.07
4.76
Standard deviation
0.03
0.06
0.16
0.03
0.06
0.15
C V (%)
5.77
5.83
3.7
5.66
5.61
3.15
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ANGâ…¡R1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ANGâ…¡R1 in samples. No significant cross-reactivity or interference between Human ANGâ…¡R1 and analogues was observed.
