The Human ANG2 (Angiopoietin-2) ELISA Kit is specifically designed for the accurate detection of Angiopoietin-2 levels in human serum, plasma, and cell culture supernatants. This advanced kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.Angiopoietin-2 is a key protein involved in angiogenesis, playing a crucial role in blood vessel formation and cell proliferation. This protein is implicated in various diseases including cancer, cardiovascular disorders, and neurodegenerative conditions, making it a valuable biomarker for studying these diseases and developing potential therapeutic interventions.
Overall, the Human ANG2 ELISA Kit provides researchers with a powerful tool for investigating the role of Angiopoietin-2 in various physiological and pathological processes, ultimately contributing to advancements in the field of biomedical research.
Product Name:
Human ANG2 (Angiopoietin 2) ELISA Kit
SKU:
HUES01295
Target:
Human ANG2 (Angiopoietin 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
28.13 pg/mL
Detection range:
46.88-3000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ANG2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ANG2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ANG2. You can calculate the concentration of Human ANG2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-105
94-110
88-101
Average (%)
99
100
95
1:4
Range (%)
96-112
83-94
97-110
Average (%)
103
88
104
1:8
Range (%)
100-117
84-99
91-107
Average (%)
108
91
98
1:16
Range (%)
96-110
87-100
95-109
Average (%)
103
92
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-109
101
EDTA plasma (n=5)
91-103
97
Cell culture media (n=5)
91-103
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
150.6
352.2
1197.3
152.9
340.9
1143.6
Standard deviation
9.2
18.7
62.3
9.0
14.7
49.2
C V (%)
6.11
5.31
5.2
5.89
4.31
4.3
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ANG2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ANG2 in samples. No significant cross-reactivity or interference between Human ANG2 and analogues was observed.
