Human Angiopoietin Like Protein 8 (ANGPTL8) ELISA Kit
The Human Angiopoietin-like protein 8 (ANGPTL8) ELISA Kit is specifically designed for the accurate detection of ANGPTL8 levels in human biological samples, including serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.ANGPTL8, also known as Betatrophin, is a critical regulator of lipid metabolism and glucose homeostasis, making it a valuable biomarker for studying metabolic disorders such as obesity, diabetes, and cardiovascular disease.
By measuring ANGPTL8 levels, researchers can gain insights into the underlying mechanisms of these conditions and potentially develop new therapeutic strategies.Overall, the Human ANGPTL8 ELISA Kit is a valuable tool for researchers seeking to explore the role of ANGPTL8 in human physiology and pathology, offering a reliable and sensitive method for quantifying ANGPTL8 levels in biological samples.
Product Name:
Human Angiopoietin Like Protein 8 (ANGPTL8) ELISA Kit
Product Code:
HUDL00178
Size:
96 Assays
Target:
ANGPTL8
Synonyms:
RIFL, TD26, C19orf80, Lipasin, Betatrophin, Hepatocellular Carcinoma-Associated Protein TD26
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
