Description
Product Name: | Human Anti-Endometrial IgG Autoantibodies ELISA Kit |
SKU: | HDES0074 |
Size: | 96T |
Target: | EM-Ab |
Detection Method: | Indirect ELISA |
Reactivity: | Human |
Result Type: | Qualitative: Sensitivity 98.4%, Specificity 99.8% |
Sample Type: | Serum |
Detection Wavelength: | 450/630nm |
Storage: | The majority of kits have a shelf-life of 12 months from the production date at 2-8℃ |
This ELISA kit uses Indirect-ELISA as the method to detect the Endomethal (EM) antibody (IgG) in human serum. The micro ELISA plate provided in this kit has been pre-coated with purified EM antigen. Samples are added to the micro ELISA plate wells and the EM-Ab in which will combine with the pre-coated antigen to form antigen-antibody compound. Free components are washed away. The HRP conjugated Mouse-anti-Human IgG is added to each well and react with the compound to form “EM antigen- EM antibody-HRP conjugate” compound. The TMB substrate is added to initiate the color developing reaction. The presence of EM -Ab can be determined according to the OD value after colorimetric assay with the Micro-plate reader.