Description
Product Name: | Human Anti-Ovary IgG Autoantibodies ELISA Kit |
SKU: | HDES0078 |
Size: | 96T |
Target: | AOA-Ab |
Detection Method: | Indirect ELISA |
Reactivity: | Human |
Result Type: | Qualitative: Sensitivity 98.1%, Specificity 99.7% |
Sample Type: | Serum |
Detection Wavelength: | 450/630nm |
Storage: | The majority of kits have a shelf-life of 12 months from the production date at 2-8℃ |
This ELISA kit uses Indirect-ELISA as the method to detect the anti-Ovary (AO) antibody (IgG) in human serum. The ELISA Microtiter plate provided in this kit has been pre-coated with purified ovary membrane antigen. Samples are added to the ELISA Microtiter plate wells and the AO-Ab in which will combine with the pre-coated antigen to form antigen-antibody compound. Free components are washed away. The HRP conjugate is added to each well and react with the compound to form “ovary membrane antigen- AO antibody-HRP conjugate” compound. The Substrate Reagent is added to initiate the color developing reaction. The presence of AO-Ab (IgG) can be determined according to the OD value after colorimetric assay with the Micro-plate reader.