The Human ANXA2 (Annexin A2) CLIA Kit is a cutting-edge diagnostic tool designed for the accurate measurement of Annexin A2 levels in human samples. This kit offers unparalleled sensitivity and specificity, guaranteeing precise and consistent results for a variety of research purposes.Annexin A2 is a key protein known for its involvement in numerous cellular processes, including membrane trafficking, cell signaling, and cytoskeletal organization.
Dysregulation of Annexin A2 has been linked to various diseases, such as cancer, autoimmune disorders, and infectious diseases, making it a valuable biomarker for understanding and potentially treating these conditions.With the Human ANXA2 CLIA Kit, researchers can uncover invaluable insights into Annexin A2 biology and its implications in human health, paving the way for innovative therapeutic strategies and personalized medicine approaches.
Product Name:
Human ANXA2 (Annexin A2) CLIA Kit
SKU:
HUES00324
Target:
Human ANXA2 (Annexin A2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANXA2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANXA2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ANXA2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ANXA2. You can calculate the concentration of Human ANXA2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-109
86-100
102-116
Average (%)
101
92
107
1:4
Range (%)
104-120
103-117
102-116
Average (%)
110
110
109
1:8
Range (%)
99-114
102-114
90-107
Average (%)
105
108
98
1:16
Range (%)
97-109
95-109
96-110
Average (%)
102
100
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
99
EDTA plasma (n=5)
93-105
99
Cell culture media (n=5)
94-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.53
1.3
3.34
0.48
1.2
3.59
Standard deviation
0.07
0.11
0.36
0.06
0.11
0.27
C V (%)
13.21
8.46
10.78
12.5
9.17
7.52
Sample type &Sample volume:
Tissue homogenates,cell lysates and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ANXA2 concentrations in Tissue homogenates,cell lysates and other biological fluids.
Specificity:
This kit recognizes Human ANXA2 in samples. No significant cross-reactivity or interference between Human ANXA2 and analogues was observed.
