The Human ANXA5 (Annexin A5) CLIA Kit is a highly sensitive and specific assay designed for the accurate measurement of annexin A5 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it an ideal tool for researchers and clinicians working in the fields of apoptosis, inflammation, and thrombosis.Annexin A5 is a key protein involved in regulating cell membrane integrity and apoptosis, making it an important biomarker for various diseases including cancer, cardiovascular disorders, and autoimmune conditions.
By accurately measuring annexin A5 levels, researchers can gain valuable insights into disease pathogenesis and potential therapeutic interventions. With its high sensitivity and specificity, the Human ANXA5 CLIA Kit is a valuable tool for studying annexin A5 biology and its role in disease progression, ultimately aiding in the development of novel diagnostic and therapeutic strategies.
Product Name:
Human ANXA5 (Annexin A5) CLIA Kit
SKU:
HUES00304
Target:
Human ANXA5 (Annexin A5)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANXA5. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANXA5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ANXA5, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ANXA5. You can calculate the concentration of Human ANXA5 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-115
94-109
100-118
Average (%)
106
101
108
1:4
Range (%)
94-109
87-101
91-102
Average (%)
100
92
96
1:8
Range (%)
92-109
88-98
86-100
Average (%)
99
93
91
1:16
Range (%)
99-115
88-102
93-109
Average (%)
107
93
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-106
99
EDTA plasma (n=5)
86-100
92
Cell culture media (n=5)
94-107
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
198.97
362.03
1960.13
204.27
395.76
1829.49
Standard deviation
24.21
33.42
214.63
23.92
32.21
140.14
C V (%)
12.17
9.23
10.95
11.71
8.14
7.66
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ANXA5 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ANXA5 in samples. No significant cross-reactivity or interference between Human ANXA5 and analogues was observed.
