The ApoB100 ELISA Kit is designed for the quantitative detection of Apolipoprotein B100 (ApoB100) levels in various human biological samples. ApoB100 is a critical component of low-density lipoproteins (LDLs) and is closely associated with cholesterol transport in the body. Its levels are significant in understanding lipid metabolism, cardiovascular health, and are often implicated in disorders like atherosclerosis and coronary artery disease. Accurate measurement of ApoB100 is essential for understanding its role in such mechanisms and for developing targeted therapeutic strategies.
Assay Genie's ApoB100 ELISA Kit offers exceptional sensitivity and specificity, ensuring reliable and reproducible results. Manufactured under stringent quality control standards, this kit provides robust performance and is easy to use, making it an excellent choice for both research and clinical applications. Trust Assay Genie's ApoB100 ELISA Kit for accurate and dependable quantification of this crucial biomarker in your studies.
Product Name:
Human ApoB100 (Apolipoprotein B100) ELISA Kit
SKU:
AEES00027
Target:
Human ApoB100 (Apolipoprotein B100)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 ng/mL
Detection range:
62.50-4000 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ApoB100. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ApoB100 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ApoB100, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ApoB100. You can calculate the concentration of Human ApoB100 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-97
84-95
85-95
Average (%)
90
90
90
1:4
Range (%)
98-114
84-97
95-110
Average (%)
104
91
101
1:8
Range (%)
93-108
81-92
93-111
Average (%)
101
86
101
1:16
Range (%)
95-110
81-93
94-110
Average (%)
102
87
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-100
92
EDTA plasma (n=5)
84-98
90
Cell culture media (n=5)
87-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
188.3
533.3
1428.9
176.2
522.6
1444.0
Standard deviation
10.7
29.9
60.0
9.3
29.3
47.7
C V (%)
5.68
5.61
4.2
5.28
5.61
3.3
Sample type &Sample volume:
serum, plasma, urine, saliva; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ApoB100 concentrations in serum, plasma, urine, saliva.
Specificity:
This kit recognizes Human ApoB100 in samples. No significant cross-reactivity or interference between Human ApoB100 and analogues was observed.
