The Human APOC1 (Apolipoprotein C1) CLIA Kit is a reliable and accurate tool for the quantitative detection of APOC1 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.APOC1 is a key protein involved in lipid metabolism and is known to play a crucial role in cardiovascular health. Dysregulation of APOC1 levels has been associated with conditions such as atherosclerosis, hyperlipidemia, and coronary artery disease, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.
With the Human APOC1 CLIA Kit, researchers can confidently measure APOC1 levels in human samples, allowing for insights into the role of this protein in cardiovascular health and its potential as a target for drug development. Trust in the accuracy and reliability of this kit for your research needs.
Product Name:
Human ApoC1 (Apolipoprotein C1) CLIA Kit
SKU:
HUES00332
Target:
Human ApoC1 (Apolipoprotein C1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ApoC1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ApoC1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ApoC1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ApoC1. You can calculate the concentration of Human ApoC1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-117
102-117
97-114
Average (%)
109
108
105
1:4
Range (%)
88-101
96-109
87-99
Average (%)
93
102
92
1:8
Range (%)
96-113
84-96
96-111
Average (%)
104
90
103
1:16
Range (%)
91-102
94-110
102-116
Average (%)
97
101
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-101
92
EDTA plasma (n=5)
85-100
92
Cell culture media (n=5)
96-107
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.83
10.25
39.13
5.23
10.57
37.66
Standard deviation
0.47
0.86
3.76
0.64
1.1
3.91
C V (%)
9.73
8.39
9.61
12.24
10.41
10.38
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ApoC1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ApoC1 in samples. No significant cross-reactivity or interference between Human ApoC1 and analogues was observed.
