The Human ApoC2 (Apolipoprotein C2) CLIA Kit is a highly reliable tool for the quantitative detection of Apolipoprotein C2 levels in human serum, plasma, and cell culture supernatants. With superior sensitivity and specificity, this kit ensures accurate and reproducible results, making it perfect for a variety of research applications.Apolipoprotein C2 is a critical component involved in lipid metabolism and transport, playing a key role in regulating triglyceride metabolism and lipoprotein lipase activity.
Dysregulation of Apolipoprotein C2 levels is associated with various metabolic disorders, including hypertriglyceridemia and cardiovascular disease, making it a valuable biomarker for studying these conditions and developing therapeutic interventions.The Human ApoC2 CLIA Kit from Assay Genie offers researchers a powerful tool to investigate the role of Apolipoprotein C2 in health and disease, providing accurate and reliable data for a deeper understanding of lipid metabolism and related disorders.
Product Name:
Human ApoC2 (Apolipoprotein C2) CLIA Kit
SKU:
HUES00333
Target:
Human ApoC2 (Apolipoprotein C2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ApoC2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ApoC2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ApoC2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ApoC2. You can calculate the concentration of Human ApoC2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-114
85-97
90-100
Average (%)
108
92
95
1:4
Range (%)
98-112
92-110
96-109
Average (%)
105
100
101
1:8
Range (%)
103-120
102-117
88-103
Average (%)
110
109
95
1:16
Range (%)
98-110
88-101
91-104
Average (%)
104
96
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
96
EDTA plasma (n=5)
102-116
108
Cell culture media (n=5)
92-104
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.33
7.99
19.98
2.28
8.62
19.85
Standard deviation
0.24
0.82
1.34
0.25
0.66
1.5
C V (%)
10.3
10.26
6.71
10.96
7.66
7.56
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ApoC2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ApoC2 in samples. No significant cross-reactivity or interference between Human ApoC2 and analogues was observed.
