The Human APOC2 (Apolipoprotein C2) ELISA Kit is specifically designed for the precise measurement of apolipoprotein C2 levels in human serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and specificity, ensuring accurate and consistent results, making it suitable for a wide range of research purposes.Apolipoprotein C2 is a vital protein involved in lipid metabolism, specifically in the regulation of triglyceride levels. Dysregulation of apolipoprotein C2 has been associated with various metabolic disorders such as hyperlipidemia and cardiovascular diseases.
Thus, it serves as a crucial biomarker for investigating these conditions and developing potential therapeutic interventions.With the Human APOC2 ELISA Kit, researchers can confidently study the role of apolipoprotein C2 in lipid metabolism and its implications in various metabolic disorders, ultimately advancing our understanding of these conditions and opening avenues for novel treatment strategies.
Product Name:
Human ApoC2 (Apolipoprotein C2) ELISA Kit
SKU:
HUES01671
Target:
Human ApoC2 (Apolipoprotein C2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ApoC2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ApoC2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ApoC2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ApoC2. You can calculate the concentration of Human ApoC2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-107
99-110
95-108
Average (%)
100
105
102
1:4
Range (%)
99-116
87-100
98-111
Average (%)
107
93
104
1:8
Range (%)
95-108
82-93
99-111
Average (%)
101
86
105
1:16
Range (%)
99-112
83-97
97-113
Average (%)
105
88
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-97
90
EDTA plasma (n=5)
90-102
97
Cell culture media (n=5)
86-96
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
11
19.5
83.6
12.1
19.9
86.3
Standard deviation
0.8
0.8
3.9
0.8
0.9
2.9
C V (%)
7.27
4.1
4.67
6.61
4.52
3.36
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ApoC2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ApoC2 in samples. No significant cross-reactivity or interference between Human ApoC2 and analogues was observed.
