The Human ApoC3 (Apolipoprotein C3) CLIA Kit is designed for the accurate detection of Apolipoprotein C3 levels in human serum, plasma, and cell culture supernatants. This kit features high sensitivity and specificity, ensuring reliable and reproducible results, making it ideal for a wide range of research applications.Apolipoprotein C3 is a vital protein involved in lipid metabolism, playing a crucial role in conditions such as hypertriglyceridemia and cardiovascular diseases.
Monitoring ApoC3 levels can provide valuable insights into lipid metabolism disorders and help in the development of potential therapies.This CLIA kit offers a simple and efficient way to quantify Apolipoprotein C3 levels in various sample types, making it a valuable tool for researchers studying lipid metabolism and related health conditions.
Product Name:
Human ApoC3 (Apolipoprotein C3) CLIA Kit
SKU:
HUES00334
Target:
Human ApoC3 (Apolipoprotein C3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ApoC3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ApoC3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ApoC3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ApoC3. You can calculate the concentration of Human ApoC3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-117
91-104
98-114
Average (%)
110
96
105
1:4
Range (%)
92-107
102-118
101-117
Average (%)
99
108
108
1:8
Range (%)
93-106
88-98
98-115
Average (%)
99
93
105
1:16
Range (%)
92-103
99-117
89-100
Average (%)
98
107
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-111
102
EDTA plasma (n=5)
89-100
95
Cell culture media (n=5)
86-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
242.0
570.44
1993.54
228.02
526.25
1947.55
Standard deviation
22.43
55.56
227.46
26.86
42.99
123.09
C V (%)
9.27
9.74
11.41
11.78
8.17
6.32
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ApoC3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ApoC3 in samples. No significant cross-reactivity or interference between Human ApoC3 and analogues was observed.
