The Human ApoC4 (Apolipoprotein C4) CLIA Kit is specifically designed for the precise measurement of Apolipoprotein C4 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.Apolipoprotein C4 is a critical component of lipoprotein particles and plays a key role in lipid metabolism and transport. Its levels are associated with various metabolic disorders, including cardiovascular diseases and hyperlipidemia.
Studying Apolipoprotein C4 levels can provide valuable insights into these conditions and aid in the development of potential therapeutic strategies.Overall, the Human ApoC4 CLIA Kit is a valuable tool for researchers interested in understanding the role of Apolipoprotein C4 in health and disease, offering reliable and reproducible results for impactful research outcomes.
Product Name:
Human ApoC4 (Apolipoprotein C4) CLIA Kit
SKU:
HUES00335
Target:
Human ApoC4 (Apolipoprotein C4)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ApoC4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ApoC4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ApoC4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ApoC4. You can calculate the concentration of Human ApoC4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-102
90-105
85-100
Average (%)
95
97
92
1:4
Range (%)
88-102
92-106
92-106
Average (%)
95
99
98
1:8
Range (%)
102-119
97-113
84-98
Average (%)
110
104
91
1:16
Range (%)
89-105
87-101
86-99
Average (%)
97
94
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-111
101
EDTA plasma (n=5)
97-110
103
Cell culture media (n=5)
98-116
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.28
58.53
193.77
24.87
56.91
197.83
Standard deviation
2.82
6.46
11.66
2.18
4.23
21.19
C V (%)
11.16
11.04
6.02
8.77
7.43
10.71
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ApoC4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ApoC4 in samples. No significant cross-reactivity or interference between Human ApoC4 and analogues was observed.
