The Human APOD (Apolipoprotein D) ELISA Kit is specifically designed for the accurate measurement of apolipoprotein D levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for various research purposes.Apolipoprotein D is a key protein involved in lipid transportation and metabolism, playing a vital role in various physiological processes.
Its dysregulation has been associated with conditions such as Alzheimer's disease, schizophrenia, and cancer, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.Overall, the Human APOD ELISA Kit is a reliable tool for researchers looking to investigate the role of apolipoprotein D in health and disease, providing valuable insights into its implications and potential therapeutic targets.
Product Name:
Human ApoD (Apolipoprotein D) ELISA Kit
SKU:
HUES01674
Target:
Human ApoD (Apolipoprotein D)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ApoD. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ApoD and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ApoD, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ApoD. You can calculate the concentration of Human ApoD in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-108
95-110
94-110
Average (%)
101
103
100
1:4
Range (%)
94-110
85-98
97-113
Average (%)
101
92
104
1:8
Range (%)
98-113
86-97
94-111
Average (%)
105
91
102
1:16
Range (%)
96-108
80-93
91-103
Average (%)
102
86
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-98
92
EDTA plasma (n=5)
84-98
91
Cell culture media (n=5)
91-105
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.1
5.0
15.5
2.0
4.9
16.2
Standard deviation
0.1
0.2
0.6
0.1
0.3
0.6
C V (%)
4.76
4.0
3.87
5.0
6.12
3.7
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ApoD concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ApoD in samples. No significant cross-reactivity or interference between Human ApoD and analogues was observed.
