The Human ApoF (Apolipoprotein F) CLIA Kit is a reliable and accurate tool designed for the quantitative measurement of Apolipoprotein F levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for various research applications.Apolipoprotein F is a key protein involved in lipid metabolism and regulation, playing a crucial role in lipid transport and homeostasis.
Dysregulation of Apolipoprotein F is associated with various metabolic disorders and cardiovascular diseases, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.Overall, the Human ApoF CLIA Kit provides researchers with a powerful tool to investigate the role of Apolipoprotein F in health and disease, offering valuable insights into potential diagnostic and therapeutic strategies.
Product Name:
Human ApoF (Apolipoprotein F) CLIA Kit
SKU:
HUES00338
Target:
Human ApoF (Apolipoprotein F)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ApoF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ApoF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ApoF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ApoF. You can calculate the concentration of Human ApoF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-97
85-98
89-104
Average (%)
91
91
96
1:4
Range (%)
99-113
100-114
92-107
Average (%)
105
108
98
1:8
Range (%)
95-106
88-103
96-109
Average (%)
100
95
104
1:16
Range (%)
98-113
96-110
90-101
Average (%)
104
103
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-99
92
EDTA plasma (n=5)
90-102
95
Cell culture media (n=5)
85-101
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
99.93
230.64
749.83
100.77
218.82
783.73
Standard deviation
10.16
16.17
71.98
8.22
22.12
63.01
C V (%)
10.17
7.01
9.6
8.16
10.11
8.04
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ApoF concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ApoF in samples. No significant cross-reactivity or interference between Human ApoF and analogues was observed.
