The Human ApoF (Apolipoprotein F) ELISA Kit is expertly designed to enable the precise quantitative detection of ApoF levels in diverse human biological samples.
Apolipoprotein F is a key protein involved in lipid metabolism and plays a significant role in lipid transportation and cholesterol metabolism in the body. ApoF is vital in regulating lipid homeostasis and contributes to the proper functioning of lipoproteins. Accurate measurement of ApoF levels is crucial for gaining insights into lipid metabolism regulation and its potential impact on cardiovascular health. This ELISA kit offers exceptional sensitivity and specificity, providing accurate and reproducible results. With stringent quality control measures and robust performance, this kit ensures reliable data for both research and clinical applications. Rely on the Assay Genie's ApoF ELISA Kit for the precise and dependable quantification of this essential biomarker in your studies.
Product Name:
Human ApoF (Apolipoprotein F) ELISA Kit
SKU:
AEES00076
Target:
Human ApoF (Apolipoprotein F)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ApoF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ApoF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ApoF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ApoF. You can calculate the concentration of Human ApoF in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-106
93-103
91-99
Average (%)
100
97
96
1:4
Range (%)
88-100
89-101
99-109
Average (%)
94
94
102
1:8
Range (%)
85-99
94-109
91-102
Average (%)
93
101
97
1:16
Range (%)
90-102
87-95
94-104
Average (%)
97
91
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
82-93
90
EDTA plasma (n=5)
94-103
100
Cell culture media (n=5)
91-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.17
5.23
23.43
2.42
5.05
22.81
Standard deviation
0.12
0.26
1.25
0.13
0.25
1.02
C V (%)
5.53
4.97
5.34
5.37
4.95
4.47
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ApoF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ApoF in samples. No significant cross-reactivity or interference between Human ApoF and analogues was observed.
