The Human ApoH (Apolipoprotein H) CLIA Kit is a cutting-edge assay designed to accurately measure apolipoprotein H levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results for a variety of research applications.Apolipoprotein H is a crucial protein in lipid metabolism and plays a key role in the transport of cholesterol and other lipids in the bloodstream.
Dysregulation of apolipoprotein H levels has been linked to various diseases such as cardiovascular disorders, diabetes, and Alzheimer's disease, making it an important biomarker for studying these conditions and potential therapeutic interventions.Overall, the Human ApoH CLIA Kit offers researchers a powerful tool for investigating the role of apolipoprotein H in health and disease, providing valuable insights into its potential as a therapeutic target.
Product Name:
Human ApoH (Apolipoprotein H) CLIA Kit
SKU:
HUES00339
Target:
Human ApoH (Apolipoprotein H)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ApoH. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ApoH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ApoH, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ApoH. You can calculate the concentration of Human ApoH in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-100
101-115
95-108
Average (%)
91
109
102
1:4
Range (%)
97-110
93-108
84-97
Average (%)
104
99
91
1:8
Range (%)
90-105
103-116
87-103
Average (%)
96
109
94
1:16
Range (%)
95-108
94-110
100-117
Average (%)
101
100
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-101
94
EDTA plasma (n=5)
88-100
93
Cell culture media (n=5)
94-111
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
247.79
574.87
1815.52
242.73
613.72
1782.4
Standard deviation
30.08
59.44
204.79
26.34
45.11
159.35
C V (%)
12.14
10.34
11.28
10.85
7.35
8.94
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ApoH concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ApoH in samples. No significant cross-reactivity or interference between Human ApoH and analogues was observed.
