The Human Apolipoprotein F (ApoF) ELISA Kit from AssayGenie is a powerful tool for the accurate measurement of Apolipoprotein F levels in human serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.Apolipoprotein F is a key protein involved in lipid metabolism and the regulation of cholesterol levels in the body. It plays a crucial role in cardiovascular health and is associated with conditions such as atherosclerosis and coronary artery disease.
By measuring Apolipoprotein F levels, researchers can gain valuable insights into these conditions and potentially develop new therapeutic strategies.With its user-friendly format and high-quality components, the Human Apolipoprotein F (ApoF) ELISA Kit is an essential tool for scientists and researchers working in the fields of lipid biology, cardiovascular research, and metabolic disorders. Trust AssayGenie for accurate and reliable results in your Apolipoprotein F studies.
Product Name:
Human Apolipoprotein F (APOF) ELISA Kit
Product Code:
HUDL00219
Size:
96 Assays
Target:
APOF
Synonyms:
Apo-F, LTIP, Lipid transfer inhibitor protein
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
