The Human APOM (Apolipoprotein M) CLIA Kit is expertly designed for precise measurement of apolipoprotein M levels in human samples such as serum, plasma, and cell culture supernatants. With exceptional accuracy and consistency, this kit delivers dependable results, making it an invaluable tool for various research studies.Apolipoprotein M is a key protein involved in lipid metabolism and transportation, playing a crucial role in cardiovascular health.
Its levels have been linked to conditions such as atherosclerosis, diabetes, and metabolic disorders, making it a valuable biomarker for understanding these diseases and exploring potential treatment options.Overall, the Human APOM CLIA Kit offers reliable and sensitive detection of apolipoprotein M, making it an essential asset for researchers looking to study lipid metabolism and its implications on human health.
Product Name:
Human ApoM (Apolipoprotein M) CLIA Kit
SKU:
HUES00340
Target:
Human ApoM (Apolipoprotein M)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ApoM. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ApoM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ApoM, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ApoM. You can calculate the concentration of Human ApoM in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-105
90-106
91-102
Average (%)
100
98
97
1:4
Range (%)
91-106
100-112
96-110
Average (%)
98
105
101
1:8
Range (%)
86-100
90-106
88-103
Average (%)
93
97
94
1:16
Range (%)
95-110
96-111
97-111
Average (%)
102
103
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-114
104
EDTA plasma (n=5)
90-105
97
Cell culture media (n=5)
98-111
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
229.43
473.1
2441.35
237.91
509.2
2261.9
Standard deviation
28.01
38.6
216.55
24.2
41.75
232.07
C V (%)
12.21
8.16
8.87
10.17
8.2
10.26
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ApoM concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ApoM in samples. No significant cross-reactivity or interference between Human ApoM and analogues was observed.
