The Human AQP-3 (Aquaporin 3) CLIA Kit is a state-of-the-art assay designed for the precise measurement of AQP-3 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.Aquaporin 3, also known as AQP-3, is a vital membrane protein that facilitates the transport of water and glycerol across cell membranes. Its role in maintaining skin hydration and barrier function has led to its significance in skin disorders, including psoriasis and atopic dermatitis.
Furthermore, AQP-3 has been implicated in regulating water homeostasis in various tissues, making it a valuable target for studying conditions such as kidney disease and metabolic disorders.Overall, the Human AQP-3 CLIA Kit provides researchers with a powerful tool for investigating the role of Aquaporin 3 in various physiological and pathological processes, paving the way for potential therapeutic interventions in related diseases.
Product Name:
Human AQP-3 (Aquaporin 3) CLIA Kit
SKU:
HUES00351
Target:
Human AQP-3 (Aquaporin 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human AQP-3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AQP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AQP-3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human AQP-3. You can calculate the concentration of Human AQP-3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-98
96-108
94-109
Average (%)
91
102
100
1:4
Range (%)
101-113
88-100
87-104
Average (%)
106
93
95
1:8
Range (%)
86-99
86-99
87-99
Average (%)
93
91
93
1:16
Range (%)
100-115
95-109
92-106
Average (%)
106
101
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-113
104
EDTA plasma (n=5)
94-107
101
Cell culture media (n=5)
87-99
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
257.3
592.15
2128.75
259.71
623.53
1968.13
Standard deviation
26.27
47.19
132.41
27.48
73.45
190.91
C V (%)
10.21
7.97
6.22
10.58
11.78
9.7
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human AQP-3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human AQP-3 in samples. No significant cross-reactivity or interference between Human AQP-3 and analogues was observed.
