The Human ARG-1 (Arginase I) CLIA Kit is developed for the precise measurement of arginase I levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research purposes.Arginase I is a key enzyme involved in the urea cycle, breaking down arginine into ornithine and urea. Dysregulation of arginase I has been linked to various diseases, such as cardiovascular disorders, liver diseases, and immune system dysfunction.
Monitoring arginase I levels can provide valuable insights into these conditions and aid in the development of potential therapeutic interventions.Overall, the Human ARG-1 CLIA Kit is a valuable tool for researchers studying arginase I biology and its role in disease pathology, offering a reliable and efficient method for quantifying arginase I levels in human samples.
Product Name:
Human Arg 1 (Arginase I) CLIA Kit
SKU:
HUES00354
Target:
Human Arg 1 (Arginase I)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human Arg. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Arg and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Arg, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human Arg. You can calculate the concentration of Human Arg in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-111
88-100
96-110
Average (%)
103
93
104
1:4
Range (%)
92-106
93-105
97-111
Average (%)
99
99
105
1:8
Range (%)
101-117
86-99
92-102
Average (%)
108
93
97
1:16
Range (%)
97-114
101-118
95-106
Average (%)
104
108
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-101
93
EDTA plasma (n=5)
91-104
96
Cell culture media (n=5)
90-105
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.65
7.01
23.8
2.63
7.39
22.27
Standard deviation
0.24
0.74
2.35
0.3
0.74
1.61
C V (%)
9.06
10.56
9.87
11.41
10.01
7.23
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human Arg concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Arg in samples. No significant cross-reactivity or interference between Human Arg and analogues was observed.
