Human ARL1 (Aldose Reductase Like Protein 1) ELISA Kit
The Human ARL1 (Aldose Reductase-like Protein 1) ELISA Kit allows for the precise measurement of ARL1 levels in human samples including serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit offers accurate and reproducible results, making it a valuable tool for various research applications.ARL1, a protein similar to aldose reductase, is involved in various cellular processes and has been implicated in diseases such as cancer, cardiovascular disorders, and neurodegenerative conditions.
By detecting ARL1 levels, researchers can gain insights into disease mechanisms and potential therapeutic targets, making this ELISA kit essential for understanding these complex diseases and developing new treatments.
Product Name:
Human ARL1 (Aldose Reductase Like Protein 1) ELISA Kit
SKU:
HUES03446
Target:
Human ARL1 (Aldose Reductase Like Protein 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ARL1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ARL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ARL1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ARL1. You can calculate the concentration of Human ARL1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-107
92-105
86-102
Average (%)
98
99
93
1:4
Range (%)
88-101
82-93
88-100
Average (%)
94
87
94
1:8
Range (%)
88-99
84-97
85-96
Average (%)
93
89
90
1:16
Range (%)
89-102
81-94
84-98
Average (%)
96
86
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-97
91
EDTA plasma (n=5)
90-103
95
Cell culture media (n=5)
85-97
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
235.28
603.52
1722.01
249.22
662.01
1781.93
Standard deviation
13.72
34.04
76.11
12.61
30.25
83.39
C V (%)
5.83
5.64
4.42
5.06
4.57
4.68
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ARL1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ARL1 in samples. No significant cross-reactivity or interference between Human ARL1 and analogues was observed.
