The Human ARR Beta2 (Arrestin Beta 2) CLIA Kit is specifically designed for the accurate measurement of ARR Beta2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.ARR Beta2 is a vital protein involved in regulating G-protein coupled receptor signaling and plays a crucial role in various cellular processes. Dysregulation of ARR Beta2 has been associated with diseases such as cancer, cardiovascular disorders, and neurodegenerative conditions, highlighting its importance as a biomarker for researching these diseases and developing potential treatments.
Overall, the Human ARR Beta2 CLIA Kit from Assay Genie provides researchers with a valuable tool for studying the role of ARR Beta2 in health and disease, with the accuracy and precision needed for meaningful results in their experiments.
Product Name:
Human ARR beta2 (Arrestin Beta 2) CLIA Kit
SKU:
HUES00358
Target:
Human ARR beta2 (Arrestin Beta 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ARRβ2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ARRβ2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ARRβ2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ARRβ2. You can calculate the concentration of Human ARRβ2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-116
97-112
95-108
Average (%)
107
105
100
1:4
Range (%)
94-111
95-110
91-106
Average (%)
102
101
99
1:8
Range (%)
89-99
101-117
96-110
Average (%)
94
108
103
1:16
Range (%)
95-110
102-119
98-109
Average (%)
102
109
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-96
91
EDTA plasma (n=5)
98-114
104
Cell culture media (n=5)
99-115
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
238.45
558.37
2292.39
257.41
539.98
2333.7
Standard deviation
24.66
43.89
209.98
31.89
63.88
223.34
C V (%)
10.34
7.86
9.16
12.39
11.83
9.57
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ARRβ2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ARRβ2 in samples. No significant cross-reactivity or interference between Human ARRβ2 and analogues was observed.
