Human Aryl Hydrocarbon Receptor Repressor (AHRR) ELISA Kit
The Human Aryl Hydrocarbon Receptor Repressor (AHRR) ELISA Kit from Assay Genie is a reliable and sensitive assay designed for the accurate detection of AHRR levels in human serum, plasma, and cell culture supernatants. This kit offers high specificity and sensitivity, ensuring precise and consistent results for a variety of research applications.AHRR is a key regulator of the aryl hydrocarbon receptor pathway, playing a critical role in the detoxification of harmful environmental pollutants and carcinogens.
Dysregulation of AHRR has been implicated in a range of health conditions, including cancer, immune disorders, and developmental abnormalities, making it a valuable biomarker for studying these diseases and exploring potential therapeutic interventions.Trust the Assay Genie Human AHRR ELISA Kit for robust and dependable measurement of AHRR levels in your samples, enabling insightful research and discovery in the field of environmental health and toxicology.
Product Name:
Human Aryl Hydrocarbon Receptor Repressor (AHRR) ELISA Kit
Product Code:
HUDL00117
Size:
96 Assays
Target:
AHRR
Synonyms:
AHH, AHHR, BHLHE77, Aryl Hydrocarbon Receptor Regulator, Class E basic helix-loop-helix protein 77
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
