Human ASGR1 (Asialoglycoprotein Receptor 1) CLIA Kit
The Human ASGR1 (Asialoglycoprotein Receptor 1) CLIA Kit is a highly sensitive and specific assay designed for the accurate measurement of ASGR1 levels in human biological samples. This kit is ideal for researchers studying liver function, autoimmune diseases, and metabolic disorders, as ASGR1 is a key receptor involved in glycoprotein metabolism and clearance.ASGR1 plays a crucial role in the recognition and clearance of circulating glycoproteins, making it a valuable biomarker for monitoring liver function and diagnosing liver diseases.
With its precise and reliable results, this kit is suitable for a wide range of research applications and drug development studies.Overall, the Human ASGR1 CLIA Kit offers researchers a powerful tool for studying ASGR1 expression and function in various physiological and pathological conditions, ultimately advancing our understanding of liver biology and disease mechanisms.
Product Name:
Human ASGR1 (Asialoglycoprotein Receptor 1) CLIA Kit
SKU:
HUES00363
Target:
Human ASGR1 (Asialoglycoprotein Receptor 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ASGR1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ASGR1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ASGR1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ASGR1. You can calculate the concentration of Human ASGR1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
103-117
99-113
94-105
Average (%)
110
106
100
1:4
Range (%)
87-101
91-102
90-106
Average (%)
94
97
98
1:8
Range (%)
90-103
100-116
91-105
Average (%)
97
106
98
1:16
Range (%)
91-104
95-109
89-101
Average (%)
97
103
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-108
100
EDTA plasma (n=5)
95-106
100
Cell culture media (n=5)
98-111
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
97.51
248.1
917.17
90.71
252.0
1004.45
Standard deviation
11.22
25.08
65.21
9.27
28.17
106.87
C V (%)
11.51
10.11
7.11
10.22
11.18
10.64
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ASGR1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ASGR1 in samples. No significant cross-reactivity or interference between Human ASGR1 and analogues was observed.
