Human ASGR2 (Asialoglycoprotein Receptor 2) CLIA Kit
The Human ASGR2 (Asialoglycoprotein Receptor 2) CLIA Kit is a state-of-the-art assay designed for the precise detection of ASGR2 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.ASGR2, also known as the Asialoglycoprotein Receptor 2, plays a critical role in the clearance of circulating glycoproteins from the bloodstream.
Dysregulation of ASGR2 has been linked to various liver disorders and metabolic conditions, making it a valuable biomarker for studying these diseases and potentially developing targeted therapies.With the Human ASGR2 CLIA Kit, researchers can confidently measure ASGR2 levels and gain valuable insights into its role in health and disease. Trust in this advanced assay for reliable and reproducible results in your research endeavors.
Product Name:
Human ASGR2 (Asialoglycoprotein Receptor 2) CLIA Kit
SKU:
HUES00362
Target:
Human ASGR2 (Asialoglycoprotein Receptor 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ASGR2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ASGR2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ASGR2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ASGR2. You can calculate the concentration of Human ASGR2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-98
96-108
94-104
Average (%)
93
102
99
1:4
Range (%)
100-114
84-96
86-101
Average (%)
105
90
93
1:8
Range (%)
103-116
88-101
86-99
Average (%)
110
93
93
1:16
Range (%)
94-106
95-113
90-102
Average (%)
99
103
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-98
92
EDTA plasma (n=5)
97-110
104
Cell culture media (n=5)
87-99
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
107.2
274.5
820.21
98.79
272.56
787.99
Standard deviation
13.36
26.0
59.88
10.34
32.46
47.59
C V (%)
12.46
9.47
7.3
10.47
11.91
6.04
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ASGR2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ASGR2 in samples. No significant cross-reactivity or interference between Human ASGR2 and analogues was observed.
