The Human ASPN (Asporin) CLIA Kit is a powerful tool for the precise measurement of asporin levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.Asporin is a key protein involved in regulating bone formation and cartilage development, playing a crucial role in conditions such as osteoarthritis and other musculoskeletal disorders.
By accurately measuring asporin levels, researchers can gain valuable insights into these conditions and potentially identify new therapeutic targets.Overall, the Human ASPN CLIA Kit is a valuable tool for researchers studying musculoskeletal disorders and looking to better understand the role of asporin in disease progression and treatment.
Product Name:
Human ASPN (Asporin) CLIA Kit
SKU:
HUES00367
Target:
Human ASPN (Asporin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ASPN. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ASPN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ASPN, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ASPN. You can calculate the concentration of Human ASPN in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-112
97-108
85-101
Average (%)
105
102
92
1:4
Range (%)
88-102
102-118
95-108
Average (%)
93
108
101
1:8
Range (%)
96-112
90-104
84-98
Average (%)
102
97
90
1:16
Range (%)
96-112
85-96
99-114
Average (%)
104
91
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-106
99
EDTA plasma (n=5)
85-100
92
Cell culture media (n=5)
85-100
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
213.72
390.01
1872.48
207.67
418.18
1970.72
Standard deviation
19.13
40.8
178.63
17.88
49.01
132.43
C V (%)
8.95
10.46
9.54
8.61
11.72
6.72
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ASPN concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ASPN in samples. No significant cross-reactivity or interference between Human ASPN and analogues was observed.
