Human Astacin Like Metallo Endopeptidase (ASTL) ELISA Kit
The Human ASTL (Astacin-like metallo-endopeptidase ASTL) ELISA Kit is a specialized tool used for the precise measurement of ASTL levels in human samples including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it a valuable asset for various research purposes.ASTL, a member of the astacin family of metalloendopeptidases, plays a critical role in various biological processes such as extracellular matrix remodeling, tissue development, and immune response regulation.
Dysregulation of ASTL is associated with a range of diseases including cancer, inflammation, and cardiovascular disorders, highlighting its importance as a potential biomarker for disease diagnosis and treatment monitoring.By utilizing the Human ASTL ELISA Kit, researchers can gain valuable insights into the role of ASTL in health and disease, paving the way for advancements in therapeutic strategies and personalized medicine. This kit is a reliable and efficient tool for studying the complex mechanisms of ASTL and its implications in various pathological conditions.
Product Name:
Human Astacin Like Metallo Endopeptidase (ASTL) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
