Human ATF4 (Activating Transcription Factor 4) ELISA Kit
The Human ATF4 (Activating Transcription Factor 4) ELISA Kit is specifically designed for the precise measurement of ATF4 levels in human serum, plasma, and cell culture supernatants. This ELISA kit is characterized by its high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.ATF4 is a key transcription factor that plays a critical role in cellular response to stress and regulates the expression of genes involved in processes such as amino acid metabolism, apoptosis, and oxidative stress.
Dysregulation of ATF4 has been implicated in various diseases, including cancer, diabetes, and neurodegenerative disorders, highlighting its importance as a biomarker in disease research and drug development.Overall, the Human ATF4 ELISA Kit from Assay Genie provides researchers with a valuable tool for studying ATF4-mediated pathways and potential therapeutic targets in various disease conditions.
Product Name:
Human ATF4 (Activating Transcription Factor 4) ELISA Kit
SKU:
HUES01720
Target:
Human ATF4 (Activating Transcription Factor 4)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ATF4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ATF4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ATF4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ATF4. You can calculate the concentration of Human ATF4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-109
94-106
92-107
Average (%)
104
99
98
1:4
Range (%)
91-103
86-101
82-94
Average (%)
97
92
87
1:8
Range (%)
91-105
84-99
88-103
Average (%)
98
90
94
1:16
Range (%)
93-107
80-92
82-95
Average (%)
100
85
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-101
93
EDTA plasma (n=5)
86-99
91
Cell culture media (n=5)
94-110
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.53
0.86
4.11
0.53
0.92
3.95
Standard deviation
0.03
0.04
0.21
0.03
0.05
0.17
C V (%)
5.66
4.65
5.11
5.66
5.43
4.3
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ATF4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ATF4 in samples. No significant cross-reactivity or interference between Human ATF4 and analogues was observed.
