Human ATP Binding Cassette Transporter A4 (ABCA4) ELISA Kit
The Human ATP-binding cassette transporter A4 (ABCA4) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of ABCA4 levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it suitable for a wide range of research applications.ABCA4 is a key protein involved in the transport of molecules across cell membranes, particularly in the retina where it plays a crucial role in the removal of toxic substances to maintain vision health.
Mutations in the ABCA4 gene are associated with various retinal degenerative diseases, such as Stargardt disease, making it a valuable biomarker for studying these conditions and developing potential therapies.Overall, the Human ATP-binding cassette transporter A4 (ABCA4) ELISA Kit is a valuable tool for researchers interested in studying the role of ABCA4 in retinal health and disease, providing accurate and reliable measurements for further understanding of its function and implications in various disorders.
Product Name:
Human ATP Binding Cassette Transporter A4 (ABCA4) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
