Human ATPase, Ca++ Transporting, Plasma Membrane 2 (ATP2B2) ELISA Kit
The Human ATPase Ca++ transporting plasma membrane 2 (ATP2B2) ELISA Kit is specifically designed for the accurate detection of ATP2B2 levels in human serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, ensuring consistent and reliable results for various research applications.ATP2B2 is a critical enzyme involved in calcium transport across the plasma membrane, playing a crucial role in maintaining cellular calcium homeostasis. Dysregulation of ATP2B2 has been implicated in various diseases, including cardiovascular disorders and neurological conditions.
As such, ATP2B2 serves as an essential biomarker for understanding these diseases and developing potential therapeutic interventions.With its high-quality components and easy-to-use protocol, the Human ATPase Ca++ transporting plasma membrane 2 (ATP2B2) ELISA Kit is a valuable tool for researchers studying calcium signaling pathways and investigating the role of ATP2B2 in human health and disease.
Product Name:
Human ATPase, Ca++ Transporting, Plasma Membrane 2 (ATP2B2) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
