Human ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) ELISA Kit
The Human ATPase H+/K+ Exchanging Alpha Polypeptide (ATP4A) ELISA Kit is meticulously designed for the precise detection of ATP4A levels in human serum, plasma, and cell culture supernatants. This advanced kit boasts exceptional sensitivity and specificity, guaranteeing dependable and consistent results, thus making it exceptionally well-suited for a diverse array of research endeavors.ATP4A is a critical enzyme that plays a vital role in proton transport within cells, maintaining the acidic environment necessary for optimal cellular function.
Dysregulation of ATP4A has been linked to various health conditions, including gastric disorders and autoimmune diseases, underscoring its significance as a key biomarker for illuminating disease mechanisms and potential therapeutic interventions.Equip yourself with the cutting-edge Human ATPase H+/K+ Exchanging Alpha Polypeptide (ATP4A) ELISA Kit for unparalleled accuracy and precision in your research pursuits.
Product Name:
Human ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-98
94
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
