Human ATPase, Na+/K+ Transporting Alpha 2 Polypeptide (ATP1a2) ELISA Kit
The Human ATPase Na+/K+ Transporting Alpha 2 Polypeptide (ATP1A2) ELISA Kit is a powerful tool designed for the precise measurement of ATP1A2 levels in human samples including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it a valuable asset for a wide range of research endeavors.ATP1A2 is a critical component of the ion transport system, playing a key role in maintaining cellular function and communication. Dysregulation of ATP1A2 has been linked to various health conditions, including neurological disorders, cardiac diseases, and metabolic disorders.
As such, this ELISA kit serves as an essential tool for investigating the role of ATP1A2 in disease pathogenesis and identifying potential therapeutic targets.Don't miss out on the opportunity to enhance your research capabilities with the Human ATPase Na+/K+ Transporting Alpha 2 Polypeptide (ATP1A2) ELISA Kit. Order yours today and unlock new insights into the physiological and pathological roles of this crucial protein.
Product Name:
Human ATPase, Na+/K+ Transporting Alpha 2 Polypeptide (ATP1a2) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
