The Human ATXN1 (Ataxin-1) CLIA Kit is a highly sensitive and specific assay designed for the accurate quantification of ATXN1 levels in human serum, plasma, and cell culture supernatants. ATXN1 is a key protein involved in the development of neurodegenerative disorders such as spinocerebellar ataxia type 1 (SCA1), making it a valuable biomarker for studying these conditions and developing potential therapies.
This CLIA kit offers reliable and reproducible results, making it an essential tool for researchers investigating the role of ATXN1 in disease pathology and potential therapeutic interventions. With its high sensitivity and specificity, the Human ATXN1 CLIA Kit is ideal for a wide range of research applications in the field of neurodegenerative diseases.
Product Name:
Human ATXN1 (Ataxin 1) CLIA Kit
SKU:
HUES00369
Target:
Human ATXN1 (Ataxin 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ATXN1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ATXN1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ATXN1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ATXN1. You can calculate the concentration of Human ATXN1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-113
88-104
92-104
Average (%)
104
95
98
1:4
Range (%)
94-107
100-116
93-106
Average (%)
100
106
100
1:8
Range (%)
87-102
91-105
90-101
Average (%)
93
97
96
1:16
Range (%)
90-105
95-107
98-110
Average (%)
98
102
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-112
106
EDTA plasma (n=5)
89-102
96
Cell culture media (n=5)
95-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
193.86
428.13
1779.58
195.96
398.29
1940.34
Standard deviation
22.26
45.34
161.59
16.95
31.7
132.33
C V (%)
11.48
10.59
9.08
8.65
7.96
6.82
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ATXN1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ATXN1 in samples. No significant cross-reactivity or interference between Human ATXN1 and analogues was observed.
