Human Autophagy Related Protein 12 (ATG12) ELISA Kit
The Human Autophagy-Related Protein 12 (ATG12) ELISA Kit is a powerful tool for the precise measurement of ATG12 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.ATG12 is a key component of the autophagy pathway, which plays a critical role in maintaining cellular homeostasis by degrading damaged organelles and proteins. Dysregulation of autophagy has been implicated in various diseases, including cancer, neurodegenerative disorders, and metabolic conditions, highlighting the importance of studying ATG12 levels in these contexts.
By using the Human ATG12 ELISA Kit, researchers can gain valuable insights into the role of autophagy in disease pathogenesis and potentially identify new therapeutic targets. With its high performance and versatility, this kit is a valuable asset for any laboratory conducting research on autophagy-related processes.
Product Name:
Human Autophagy Related Protein 12 (ATG12) ELISA Kit
Product Code:
HUDL00274
Size:
96 Assays
Target:
ATG12
Synonyms:
APG12, APG12L, HAPG12, Ubiquitin-like protein ATG12
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
