The Human Axin2 (Axis Inhibition Protein 2) ELISA Kit is specifically designed for the precise detection of Axin2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.Axin2 is a key regulator in the Wnt signaling pathway, playing a critical role in cell proliferation, development, and differentiation. Dysregulation of Axin2 has been linked to various diseases including cancer, osteoporosis, and developmental disorders, highlighting its significance as a potential biomarker for disease diagnosis and therapeutic interventions.
By using the Human Axin2 ELISA Kit, researchers can uncover valuable insights into the role of Axin2 in disease pathogenesis and explore its potential as a target for novel treatment strategies. This versatile kit provides a reliable tool for studying Axin2 biology and its implications in human health and disease.
Product Name:
Human AXIN2 (Axis Inhibition Protein 2) ELISA Kit
SKU:
HUES01730
Target:
Human AXIN2 (Axis Inhibition Protein 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human AXIN2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AXIN2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AXIN2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human AXIN2. You can calculate the concentration of Human AXIN2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-107
92-105
87-99
Average (%)
100
98
92
1:4
Range (%)
91-105
86-99
84-95
Average (%)
97
91
89
1:8
Range (%)
89-99
81-92
85-98
Average (%)
94
86
91
1:16
Range (%)
91-105
80-94
87-99
Average (%)
98
87
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-106
100
EDTA plasma (n=5)
87-101
93
Cell culture media (n=5)
91-105
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.05
1.81
7.61
1.01
1.76
7.02
Standard deviation
0.06
0.09
0.29
0.07
0.09
0.27
C V (%)
5.71
4.97
3.81
6.93
5.11
3.85
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human AXIN2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human AXIN2 in samples. No significant cross-reactivity or interference between Human AXIN2 and analogues was observed.
