The Human AZU1 (Azurocidin-1) CLIA Kit is a powerful tool for accurately measuring levels of azurocidin-1 in human biological samples. This kit offers exceptional sensitivity and specificity, guaranteeing dependable and consistent results for a variety of research purposes.Azurocidin-1 is an important protein involved in innate immune responses, serving as an antimicrobial peptide and modulator of inflammation. Its dysregulation has been linked to various diseases including inflammatory disorders, infectious diseases, and cancer, highlighting its significance as a biomarker for investigating these conditions and exploring potential treatment options.
Overall, the Human AZU1 (Azurocidin-1) CLIA Kit provides researchers with a valuable tool for studying the role of azurocidin-1 in human health and disease, offering insights that may lead to advancements in diagnostics and therapeutics.
Product Name:
Human AZU1 (Azurocidin 1) CLIA Kit
SKU:
HUES00376
Target:
Human AZU1 (Azurocidin 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human AZU1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AZU1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AZU1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human AZU1. You can calculate the concentration of Human AZU1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-102
100-116
92-109
Average (%)
96
107
99
1:4
Range (%)
91-105
86-100
95-108
Average (%)
98
92
101
1:8
Range (%)
104-120
91-103
101-117
Average (%)
110
98
107
1:16
Range (%)
96-110
98-116
86-97
Average (%)
102
106
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-114
107
EDTA plasma (n=5)
94-107
100
Cell culture media (n=5)
91-105
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
98.61
283.65
896.67
89.68
275.15
946.34
Standard deviation
11.19
20.79
62.95
11.51
21.02
87.82
C V (%)
11.35
7.33
7.02
12.83
7.64
9.28
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human AZU1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human AZU1 in samples. No significant cross-reactivity or interference between Human AZU1 and analogues was observed.
